Biotechnology used to maximize reproductive efficiency on a highly genetic valued donor by increasing the number of offspring during lifetime (10 - 12 progeny/year).
- Allow access to international markets (Export - Import).
- Conserve genetics through embryo freezing.
- Produce offspring from already death or valuable sires.
- Rapid genetic selection (prove genetic merit) within a small selected population.
- Disease control.
Is a hormonal ovary stimulation treatment (FSH - LH) which induces multiple maturation and ovulation of follicles resulting in the ovary release of more than one oocyte. After superovulation, the donor cow comes in estrus (Heat) and artificial insemination (Breeding) is performed.
The process of Superovulation yields many oocytes, which are released over the course of several hours. Proper timely insemination with high quality semen is needed in order to reach a maximum number of fertilized oocytes.
After superovulation treatment, oocytes are released and fecundated in the reproductive tract becoming embryos and after 4 days they descent to the uterus. Embryos are non-surgically recovered 6-8 days after breeding though the flush of the uterus via a collection line. Embryos are cached in a filter for later search and classification on a Petri dish.
Embryo transfer is done using non-surgical embryo transfer technique. Once embryos are collected, evaluated and graded under an stereo - microscope, embryos are loaded in 0.5 cc straws and transferred in commercial bovine recipients.
The recipient cow with a previously synchronized heat is brought to the squeeze chute for corpus luteum (CL) palpation. Once CL is detected, epidural anesthesia is injected to allow easy reproductive tract manipulation. The embryo transfer gun is inserted in the mid way up the uterine horn (2d third of the horn) of the surrogate cow and then the embryo is released in the horn ipsilateral to the corpus luteum.
Conception Rate on Recipients after ET:
Conception rate directly dependents on a well balanced relation animal - nutrition - environment. Under well managed condition the producer can expect:
- Embryos transferred using fresh embryos produces a 60-75 % conception rate.
- Embryo Transfer using frozen embryos produces a 35-45 % conception rate.
Embryo Freezing (Cryopreservation)
Cryopreservation is the process of freezing embryo cells in order to preserve it for the future.
Embryos are currently frozen using one technique known as slow cooling and two different media; Embryos frozen in Ethylene Glycol (Direct Transfer) can be thawed and placed directly into the recipient cow without the use of a microscope; embryos frozen in Glycerol 10% should be thawed and rinsed through multiple washings before transferred on recipients.